APPL Rabbit mAb

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	Q9UKG1
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal Antibody
Calculated MW	79663 Da

Additional Information
Gene ID	26060
Other Names	APPL1
Dilution	WB~~1/500-1/1000 IHC-P~~N/A
Format	Liquid

Protein Information
Name	APPL1 (HGNC:24035)
Function	Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism (PubMed:10490823, PubMed:15016378, PubMed:19661063, PubMed:26073777, PubMed:26583432). Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex (PubMed:15016378). Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (By similarity). Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages (By similarity). Regulates TLR4 signaling in activated macrophages (By similarity). Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner (PubMed:26583432). Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways (PubMed:19661063, PubMed:24879834, PubMed:26073777). Required for fibroblast migration through HGF cell signaling (By similarity). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin- HDAC complex (PubMed:19433865).
Cellular Location	Early endosome membrane; Peripheral membrane protein. Nucleus. Cytoplasm. Endosome. Cell projection, ruffle {ECO:0000250\|UniProtKB:Q8K3H0}. Cytoplasmic vesicle, phagosome {ECO:0000250\|UniProtKB:Q8K3H0}. Note=Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF
Tissue Location	High levels in heart, ovary, pancreas and skeletal muscle.