ATP6V0D1 Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IP, ICC
Primary Accession	P61421
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal Antibody
Calculated MW	40329 Da

Additional Information
Gene ID	9114
Other Names	ATP6V0D1
Dilution	WB~~1/500-1/1000 IHC-P~~N/A IHC-F~~N/A IP~~N/A ICC~~N/A
Format	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Protein Information
Name	ATP6V0D1 (HGNC:13724)
Synonyms	ATP6D, VPATPD
Function	Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:28296633, PubMed:30374053, PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:30374053). May play a role in coupling of proton transport and ATP hydrolysis (By similarity). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (By similarity).
Cellular Location	Membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250\|UniProtKB:P61420}; Peripheral membrane protein. Note=Localizes to centrosome and the base of the cilium {ECO:0000250\|UniProtKB:Q6PGV1}
Tissue Location	Ubiquitous.