癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
p21 (CDKN1A) Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P, IF, FC, E |
|---|---|
| Primary Accession | P38936 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 18119 Da |
| Antigen Region | 134-164 aa |
| Gene ID | 1026 |
|---|---|
| Other Names | Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1 |
| Target/Specificity | This p21 (CDKN1A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the C-terminal region of human p21 (CDKN1A). |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 FC~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | p21 (CDKN1A) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CDKN1A (HGNC:1784) |
|---|---|
| Function | Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed:9106657). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (By similarity). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (By similarity). |
| Cellular Location | Cytoplasm. Nucleus |
| Tissue Location | Expressed in all adult tissues, with 5-fold lower levels observed in the brain |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
REFERENCES
Fukuchi, K., et al., Biochim. Biophys. Acta 1642(3):163-171 (2003).
Frouin, I., et al., J. Biol. Chem. 278(41):39265-39268 (2003).
Dupont, J., et al., J. Biol. Chem. 278(39):37256-37264 (2003).
Di Padova, M., et al., J. Biol. Chem. 278(38):36496-36504 (2003).
Bai, Y.Q., et al., Oncogene 22(39):7942-7949 (2003).
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