癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Histone H1.2 Rabbit mAb
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F |
|---|---|
| Primary Accession | P16403 |
| Reactivity | Rat, Human, Mouse |
| Host | Rabbit |
| Clonality | Monoclonal Antibody |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Purification | Affinity Purified |
| Calculated MW | 21365 Da |
| Gene ID | 3006 |
|---|---|
| Other Names | H1-2 |
| Dilution | WB~~1:1000-1:5000 IHC-P~~1:50~200 IHC-F~~N/A |
| Format | Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | H1-2 (HGNC:4716) |
|---|---|
| Function | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber (PubMed:26581166, PubMed:38530350). Histone H1-2 is required for the condensation of nucleosome chains into higher-order structured fibers (PubMed:38530350). Compared to other histone H1 variants, H1-2 plays an essential role in nucleosome condensation: its absence leads to global chromatin decompaction, which is not observed when depleting other histone H1 variants (PubMed:38530350). Histone H1-2 also acts as a histone reader: specifically recognizes and binds histone H3 trimethylated at 'lys-27' (H3K27me3) (PubMed:26581166). Histones H1 also promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex, possibly by facilitating restoration of H3K27me3 post- replication (PubMed:37429872, PubMed:40516528). Together with histone H1-3, histone H1-2 acts as a regulator of splicing, most specifically exon skipping and intron retention events: histone H1-2 has a high affinity for exons and regulates splicing by affecting RNA polymerase II (RNAPII) elongation (PubMed:37922872). Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (PubMed:38530350). |
| Cellular Location | Nucleus. Nucleus, nucleolus Chromosome. Note=Enriched at the nuclear periphery and colocalizes with compacted DNA (heterochromatin) (PubMed:26581166, PubMed:38530350). Enriched within lamina-associated domains (PubMed:38530350). |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
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