癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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DYRK1A Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q13627 |
| Other Accession | Q2TAE3, Q63470, Q61214 |
| Reactivity | Human, Mouse |
| Predicted | Mouse, Rat, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 85584 Da |
| Antigen Region | 107-136 aa |
| Gene ID | 1859 |
|---|---|
| Other Names | Dual specificity tyrosine-phosphorylation-regulated kinase 1A, Dual specificity YAK1-related kinase, HP86, Protein kinase minibrain homolog, MNBH, hMNB, DYRK1A, DYRK, MNB, MNBH |
| Target/Specificity | This DYRK1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-136 amino acids from the N-terminal region of human DYRK1A. |
| Dilution | IHC-P~~1:100~500 WB~~1:500-1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DYRK1A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DYRK1A {ECO:0000303|PubMed:25620562, ECO:0000312|HGNC:HGNC:3091} |
|---|---|
| Function | Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities (PubMed:20981014, PubMed:21127067, PubMed:23665168, PubMed:30773093, PubMed:8769099). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3 (PubMed:23665168). Plays an important role in double-strand breaks (DSBs) repair following DNA damage (PubMed:31024071). Mechanistically, phosphorylates RNF169 and increases its ability to block accumulation of TP53BP1 at the DSB sites thereby promoting homologous recombination repair (HRR) (PubMed:30773093). Also acts as a positive regulator of transcription by acting as a CTD kinase that mediates phosphorylation of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed:25620562, PubMed:29849146). May play a role in a signaling pathway regulating nuclear functions of cell proliferation (PubMed:14500717). Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Has pro- survival function and negatively regulates the apoptotic process (By similarity). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1 (By similarity). This in turn inhibits p53/TP53 activity and apoptosis (By similarity). Phosphorylates SEPTIN4, SEPTIN5 and SF3B1 at 'Thr-434' (By similarity). |
| Cellular Location | Nucleus. Nucleus speckle {ECO:0000250|UniProtKB:Q61214} |
| Tissue Location | Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DYRK1A is a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. The DYRK1A gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome.
REFERENCES
Adayev,T., Biochemistry 46 (25), 7614-7624 (2007)
Chang,H.S., Int. J. Cancer 120 (11), 2377-2385 (2007)
Alvarez,M., Mol. Biol. Cell 18 (4), 1167-1178 (2007)
Wissing,J., Mol. Cell Proteomics 6 (3), 537-547 (2007)
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