LAMP2A Rabbit mAb

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IP
Primary Accession	P13473
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Calculated MW	44961 Da

Additional Information
Gene ID	3920
Other Names	LAMP2
Dilution	WB~~1/500-1/1000 IHC-P~~N/A IP~~N/A
Format	Liquid

Protein Information
Name	LAMP2
Function	Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032, PubMed:36586411, PubMed:37390818, PubMed:8662539). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032, PubMed:36586411, PubMed:8662539). Functions by binding target proteins, such as GAPDH, NLRP3 and MLLT11, and targeting them for lysosomal degradation (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:36586411, PubMed:8662539). In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2 (PubMed:36586411). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:27628032). Required for efficient MHC class II-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHC II subunits (PubMed:15894275, PubMed:20518820). Is not required for efficient MHC class II-mediated presentation of endogenous antigens (PubMed:20518820).
Cellular Location	Lysosome membrane {ECO:0000255\|PROSITE- ProRule:PRU00740, ECO:0000269\|PubMed:11082038, ECO:0000269\|PubMed:17897319, ECO:0000269\|PubMed:18644871, ECO:0000269\|PubMed:2912382}; Single-pass type I membrane protein {ECO:0000255\|PROSITE-ProRule:PRU00740, ECO:0000269\|PubMed:17897319} Endosome membrane; Single-pass type I membrane protein {ECO:0000255\|PROSITE-ProRule:PRU00740, ECO:0000269\|PubMed:17897319}. Cell membrane; Single-pass type I membrane protein {ECO:0000255\|PROSITE-ProRule:PRU00740, ECO:0000269\|PubMed:17897319}. Cytoplasmic vesicle, autophagosome membrane {ECO:0000250\|UniProtKB:P17047}. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane
Tissue Location	Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle (PubMed:26856698, PubMed:7488019). Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver (PubMed:26856698, PubMed:7488019). Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta (PubMed:26856698).