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[KO]P70 S6 Kinase beta/SRK Rabbit mAb

Western blot analysis of S6K2 in HeLa(WT) and S6K2-Knockout HeLa(KO) using S6K2 antibody.
Immunocytochemistry analysis of S6K2 (green) in 3T3 using S6K2 antibody,and DAPI(blue).
Western blot analysis of P70 S6 Kinase beta in rat Brain, C6, Hela lysates using P70 S6 Kinase beta antibody.
Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using P70 S6 Kinase beta antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC
Primary Accession	Q9UBS0
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Purification	Affinity Purified
Calculated MW	53455 Da

Additional Information
Gene ID	6199
Other Names	RPS6KB2
Dilution	WB~~1:1000-1:5000 IHC-P~~1:50~200 FC~~1:50-1:100
Format	Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RPS6KB2
Synonyms	STK14B
Function	Phosphorylates specifically ribosomal protein S6 (PubMed:29750193). Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed:29750193).
Cellular Location	Cytoplasm. Nucleus.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Phosphorylates specifically ribosomal protein S6 (PubMed:29750193).Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed:29750193).

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¥ 1,250.00

Cat# AP75858

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[KO]P70 S6 Kinase beta/SRK Rabbit mAb

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