注册 | 登录
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   ACPP Antibody (C-term)   

ACPP Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ACPP Antibody (C-term) AP7592b
    All lanes : Anti-ACPP Antibody (C-term) at 1:500 dilution Lane 1: PC-3 whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: U-251MG whole cell lysate Lane 4: Mouse lung whole tissue lysate Lane 5: Mouse prostate whole tissue lysate Lane 5: Rat prostate whole tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ACPP Antibody (C-term) AP7592b
    Western blot analysis of anti-ACPP Antibody (C-term) (Cat.#AP7592b) in mouse lung tissue lysates (35ug/lane). ACPP(arrow) was detected using the purified Pab.
  • 14 - ACPP Antibody (C-term) AP7592b
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACPP antibody (C-term) (Cat.#AP7592b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 产品详情
  • 实验流程
  • 背景知识
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P15309
Reactivity Human, Rat, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44566 Da
Antigen Region 313-341 aa
Additional Information
Gene ID 55
Other Names Prostatic acid phosphatase, PAP, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase, PAPf39, ACPP
Target/Specificity This ACPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-341 amino acids from the C-terminal region of human ACPP.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACPP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACP3 (HGNC:125)
Synonyms ACPP
Function A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed:10506173, PubMed:15280042, PubMed:20498373, PubMed:9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed:10506173, PubMed:15280042).
Cellular Location [Isoform 1]: Secreted
Tissue Location Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

ACPP is an enzyme which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.

REFERENCES

Quintero,I.B., Cancer Res. 67 (14), 6549-6554 (2007)
Klyushnenkova,E.N., Prostate 67 (10), 1019-1028 (2007)
Johnson,J.L., Biochem. J. 391 (PT 3), 699-710 (2005)

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价