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>   首页   >   产品   >   一抗   >   癌症   >   EphB2 Antibody (C-term)   

EphB2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - EphB2 Antibody (C-term) AP7623b
    All lanes: Anti-EphB2 Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 130 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - EphB2 Antibody (C-term) AP7623b
    Western blot analysis of anti-EphB2 C-term Pab (Cat. #AP7623b) in NCI-H460 cell lysate. EphB2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • 14 - EphB2 Antibody (C-term) AP7623b
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody (C-term)(Cat.#AP7623b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 14 - EphB2 Antibody (C-term) AP7623b
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EphB2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - EphB2 Antibody (C-term) AP7623b
    Confocal immunofluorescent analysis of EphB2 Antibody (C-term)(Cat#AP7623b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
All lanes: Anti-EphB2 Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 130 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession P29323
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 117493 Da
Antigen Region 1021-1050 aa
Additional Information
Gene ID 2048
Other Names Ephrin type-B receptor 2, Developmentally-regulated Eph-related tyrosine kinase, ELK-related tyrosine kinase, EPH tyrosine kinase 3, EPH-like kinase 5, EK5, hEK5, Renal carcinoma antigen NY-REN-47, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3, EPHB2, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5
Target/Specificity This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1021-1050 amino acids from the C-terminal region of human EphB2.
Dilution WB~~1:1000
IHC-P~~1:100~500
IF~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEphB2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPHB2
Synonyms DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation (PubMed:30213874).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite
Tissue Location Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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