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Citrate Synthetase Rabbit mAb

     
  • 1 - Citrate Synthetase Rabbit mAb AP76440
    Immunocytochemistry analysis of Citrate Synthetase (green) in Hela using Citrate Synthetase antibody,and DAPI(blue).
  • 2 - Citrate Synthetase Rabbit mAb AP76440
    Western blot analysis of Citrate synthetase in U2OS, C6 lysates using Citrate synthetase antibody.
  • 8 - Citrate Synthetase Rabbit mAb AP76440
    Immunohistochemistry analysis of paraffin-embedded Human tonsil using Citrate synthetase antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P
Primary Accession O75390
Reactivity Rat, Human
Host Rabbit
Clonality Monoclonal Antibody
Isotype IgG
Conjugate Unconjugated
Purification Affinity Purified
Calculated MW 51712 Da
Additional Information
Gene ID 1431
Other Names CS
Dilution WB~~1:1000-1:5000
IHC-P~~1:50~200
Format Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Protein Information
Name CS
Function Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P00889}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947). It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

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