癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Ubiquitin Conjugation Factor E4 B Rabbit mAb
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Application 
| WB |
|---|---|
| Primary Accession | O95155 |
| Reactivity | Rat, Human, Mouse |
| Host | Rabbit |
| Clonality | Monoclonal Antibody |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Purification | Affinity Purified |
| Calculated MW | 146185 Da |
| Gene ID | 10277 |
|---|---|
| Other Names | UBE4B |
| Dilution | WB~~1:1000 |
| Format | Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | UBE4B (HGNC:12500) |
|---|---|
| Function | Acts as an E3 ubiquitin ligase and E4 ubiquitin chain elongation enzyme specifically involved in polyubiquitin chain assembly (PubMed:21317885). Plays an essential role in cardiac development and in the protection of neurons from degeneration evoked by ER stress (By similarity). Is recruited to early endosomes via interaction with the ESCRT-0 component HGS, where it ubiquitinates membrane protein cargo such as EGFR and APP to promote their sorting into multivesicular bodies and subsequent lysosomal degradation (PubMed:24344129, PubMed:32841720). Promotes MDM2-mediated polyubiquitination and subsequent degradation of the tumor suppressor TP53 (PubMed:21317885). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820). Mediates the formation of 'Lys-11'- or 'Lys-48'-linked polyubiquitin chains on TRA2A and PYCR2 and induces their degradation by the proteasome (PubMed:35669517). Thereby regulates the alternative splicing function of TRA2A (PubMed:35669517). Also regulates the mTOR signaling pathway by polyubiquitinating KLHL22, an E3 ligase that degrades the GATOR1 complex protein DEPDC5 (By similarity). Under mitochondrial depolarization, mediates the ubiquitination of NIPSNAP1, thereby promoting its interaction with autophagy receptors NDP52 and p62/SQSTM1 and its subsequent degradation via the lysosome-dependent pathway (PubMed:41596759). |
| Cellular Location | Early endosome. Cytoplasm Nucleus {ECO:0000250|UniProtKB:Q9ES00} |
| Tissue Location | Expressed in differentiated myotubes (at protein level) (PubMed:17369820). Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788). Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788). Almost undetectable in lung and spleen (PubMed:11802788) |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity).
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¥ 1,250.00
Cat# AP76759
