Huntingtin Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, FC, ICC
Primary Accession	P42858
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Immunogen	A synthesized peptide derived from human Huntingtin
Purification	Affinity Chromatography
Calculated MW	347603 Da

Additional Information
Gene ID	3064
Other Names	HTT
Dilution	WB~~1/500-1/1000 IHC-P~~N/A IF~~1:50~200 FC~~1:10~50 ICC~~N/A
Format	Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Protein Information
Name	HTT
Synonyms	HD, IT15
Function	[Huntingtin]: May play a role in microtubule-mediated transport or vesicle function.
Cellular Location	[Huntingtin]: Cytoplasm. Nucleus. Early endosome. Note=The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington disease neurons. Shuttles between cytoplasm and nucleus in a Ran GTPase- independent manner (PubMed:15654337). Recruits onto early endosomes in a Rab5- and HAP40-dependent fashion (PubMed:16476778)
Tissue Location	Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation

Research Areas