RYBP Rabbit mAb

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, FC, ICC, IP
Primary Accession	Q8N488
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Immunogen	A synthesized peptide derived from human RYBP
Purification	Affinity Chromatography
Calculated MW	24822 Da

Additional Information
Gene ID	23429
Other Names	RYBP
Dilution	WB~~1/500-1/1000 IHC-P~~1:50~200 IF~~1:50~200 FC~~1:10~50 ICC~~N/A IP~~N/A
Format	Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Protein Information
Name	RYBP
Synonyms	DEDAF, YEAF1
Function	Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA (By similarity). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed:27748911).
Cellular Location	Nucleus. Cytoplasm. Nucleus, nucleoplasm {ECO:0000250\|UniProtKB:Q8CCI5}. Note=Primarily found in the nucleus Detected in a punctate pattern likely to represent Polycomb group (PcG) bodies (By similarity). {ECO:0000250\|UniProtKB:Q8CCI5}
Tissue Location	Down-regulated in breast cancer tissues and in several breast cancer cell lines (at protein level) (PubMed:27748911) Widely expressed with highest levels in lymphoid tissues and placenta