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Methionyl tRNA Synthetase Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

Anti-MARS2 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-MARS2 Antibody (C-term) (AP7841b) in mouse testis tissue lysates (35ug/lane). MARS2(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody (C-term) (Cat.#AP7841b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q96GW9
Reactivity	Human, Mouse
Predicted	Rat, Bovine, Canine, Rabbit
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	66591 Da
Antigen Region	564-593 aa

Additional Information
Gene ID	92935
Other Names	Methionine--tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2
Target/Specificity	This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Methionyl tRNA Synthetase Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MARS2
Cellular Location	Mitochondrion matrix.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet <--> AMP + diphosphate + L-methionyl-tRNAMet The 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met). This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.

REFERENCES

Spencer,A.C., Biochemistry 43 (30), 9743-9754 (2004)

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Cat# AP7841b

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Methionyl tRNA Synthetase Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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