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MLK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MLK1 Antibody (C-term) AP7919a
    All lanes : Anti-MLK1 Antibody (C-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: SW480 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 122 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - MLK1 Antibody (C-term) AP7919a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P80192
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 121895 Da
Additional Information
Gene ID 4293
Other Names Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1, MAP3K9, MLK1, PRKE1
Target/Specificity This MLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1070-1104 amino acids from the C-terminal region of human MLK1.
Dilution WB~~1:1000
IHC-P~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMLK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP3K9
Synonyms MLK1, PRKE1
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
Tissue Location Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Research Areas

BACKGROUND

MLK1 is a MLK(MAP3K) type protein kinase. The catalytic domain of mixed-lineage kinases (MLKs) kinases have amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition to thee unusual nature of the kinase catalytic domains, MLK1 and MLK2 contain 2 leu/ile-zipper motifs and a basic sequence near their C-termini. MLK1 is a member of the neuronal apoptotic JNK/c-Jun pathway acting between Rac1/Cdc42 and MKK4 and -7 in death signaling. MLK1 expression has been documented in human epithelial tumor cell lines of colonic, breast and esophageal origin.

REFERENCES

Durkin, J.T., et al., Biochemistry 43(51):16348-16355 (2004).
Gallo, K.A., et al., J. Biol. Chem. 269(21):15092-15100 (1994).
Dorow, D.S., et al., Eur. J. Biochem. 213(2):701-710 (1993).

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