癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PI3KR4 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- 背景知识
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q99570 |
| Other Accession | NP_055417 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 153103 Da |
| Antigen Region | 53-83 aa |
| Gene ID | 30849 |
|---|---|
| Other Names | Phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase regulatory subunit 4, PI3-kinase p150 subunit, Phosphoinositide 3-kinase adaptor protein, PIK3R4 |
| Target/Specificity | This PI3KR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-83 amino acids from the N-terminal region of human PI3KR4. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PI3KR4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PIK3R4 |
|---|---|
| Synonyms | VPS15 {ECO:0000303|PubMed:23878393} |
| Function | Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). |
| Cellular Location | Late endosome. Cytoplasmic vesicle, autophagosome. Membrane; Lipid-anchor. Note=As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes. Localizes also to discrete punctae along the ciliary axoneme (By similarity) {ECO:0000250|UniProtKB:Q8VD65, ECO:0000305} |
| Tissue Location | Ubiquitously expressed. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
REFERENCES
Panaretou, C., et al., J. Biol. Chem. 272(4):2477-2485 (1997).
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