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NME7 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - NME7 Antibody (N-term) AP8084a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - NME7 Antibody (N-term) AP8084a
    Western blot analysis of NME7 (arrow) using rabbit polyclonal NME7 Antibody (V40) (Cat. #AP8084a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NME7 gene.
  • 1 - NME7 Antibody (N-term) AP8084a
    NME7 Antibody (V40) (Cat. #AP8084a) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the NME7 antibody detected the NME7 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9Y5B8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42492 Da
Antigen Region 25-55 aa
Additional Information
Gene ID 29922
Other Names Nucleoside diphosphate kinase 7, NDK 7, NDP kinase 7, nm23-H7, NME7
Target/Specificity This NME7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of human NME7.
Dilution IHC-P~~1:100~500
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNME7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NME7 (HGNC:20461)
Function Possesses an intrinsic kinase activity (PubMed:24807905). Displays 3'-5' exonuclease activity with a preference for single- stranded DNA (PubMed:16313181). Does not seem to have nucleoside diphosphate kinase activity (PubMed:16313181, PubMed:24807905). Functional component of the gamma-tubulin ring complex, implicated in the regulation of the microtubule-nucleating activity of the gamma- tubulin ring complex in centrosomes, in a kinase activity-dependent manner (PubMed:24807905). Part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating (PubMed:36191189).
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm, cytoskeleton, flagellum axoneme {ECO:0000250|UniProtKB:Q5E9Y9}. Cell projection, cilium. Note=Localizes to centrosomes through its assembly into gamma-tubulin ring complex. The centrosomal content of NME7 varies during the cell cycle, being highest in mitosis and lowest in early G1.
Tissue Location Expressed in airway epithelial cells.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

REFERENCES

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).

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