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|Application ||WB, IHC-P, E|
|Calculated MW||86700 Da|
|Other Names||Serine/threonine-protein kinase tousled-like 1, PKU-beta, Tousled-like kinase 1, TLK1, KIAA0137|
|Target/Specificity||This TLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720-750 amino acids from the C-terminal region of human TLK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TLK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.|
|Tissue Location||Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.|
Provided below are standard protocols that you may find useful for product applications.
The Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM]
Krause, D.R., et al., Oncogene 22(38):5927-5937 (2003).
Cabaniols, J.P., et al., Mol. Biol. Cell 10(12):4033-4041 (1999).
Sillje, H.H., et al., EMBO J. 18(20):5691-5702 (1999).
Yamakawa, A., et al., Gene 202 (1-2), 193-201 (1997).