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TLK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - TLK1 Antibody (C-term) AP8101b
    All lanes : Anti-TLK1 Antibody (L687) at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - TLK1 Antibody (C-term) AP8101b
    Western blot analysis of hTLK1-L687 (Cat. #AP8101b) in K562 cell line lysates (35ug/lane). TLK1 (arrow) was detected using the purified Pab.
  • 14 - TLK1 Antibody (C-term) AP8101b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9UKI8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 86700 Da
Additional Information
Gene ID 9874
Other Names Serine/threonine-protein kinase tousled-like 1, PKU-beta, Tousled-like kinase 1, TLK1, KIAA0137
Target/Specificity This TLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720-750 amino acids from the C-terminal region of human TLK1.
Dilution WB~~1:1000
IHC-P~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTLK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TLK1
Synonyms KIAA0137
Function Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
Cellular Location Nucleus
Tissue Location Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
Research Areas

BACKGROUND

The Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM]

REFERENCES

Krause, D.R., et al., Oncogene 22(38):5927-5937 (2003).
Cabaniols, J.P., et al., Mol. Biol. Cell 10(12):4033-4041 (1999).
Sillje, H.H., et al., EMBO J. 18(20):5691-5702 (1999).
Yamakawa, A., et al., Gene 202 (1-2), 193-201 (1997).

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