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>   首页   >   产品   >   一抗   >   代谢   >   AK3 Antibody (C-term H38)   

AK3 Antibody (C-term H38)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AK3 Antibody (C-term H38) AP8132b
    The anti-AK3 Pab (Cat. #AP8132b) is used in Western blot to detect AK3 in mouse kidney tissue lysate.
  • 1 - AK3 Antibody (C-term H38) AP8132b
    Western blot analysis of AK3 (arrow) using rabbit polyclonal AK3 Antibody (C-term H38) (Cat. #AP8132b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AK3 gene (Lane 2) (Origene Technologies).
  • 14 - AK3 Antibody (C-term H38) AP8132b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P27144
Other Accession Q9WUS0, Q9WUR9
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25268 Da
Antigen Region 195-223 aa
Additional Information
Gene ID 205
Other Names Adenylate kinase 4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170}, AK 4 {ECO:0000255|HAMAP-Rule:MF_03170}, 27410 {ECO:0000255|HAMAP-Rule:MF_03170}, 2746 {ECO:0000255|HAMAP-Rule:MF_03170}, Adenylate kinase 3-like {ECO:0000255|HAMAP-Rule:MF_03170}, GTP:AMP phosphotransferase AK4 {ECO:0000255|HAMAP-Rule:MF_03170}, AK4 {ECO:0000255|HAMAP-Rule:MF_03170}
Target/Specificity This AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the C-terminal region of human AK3.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAK3 Antibody (C-term H38) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AK4 (HGNC:363)
Function Broad-specificity mitochondrial nucleoside phosphate kinase involved in cellular nucleotide homeostasis by catalyzing nucleoside- phosphate interconversions (PubMed:19073142, PubMed:19766732, PubMed:23416111, PubMed:24767988). Similar to other adenylate kinases, preferentially catalyzes the phosphorylation of the nucleoside monophosphate AMP with ATP as phosphate donor to produce ADP (PubMed:19766732). Phosphorylates only AMP when using GTP as phosphate donor (PubMed:19766732). In vitro, can also catalyze the phosphorylation of CMP, dAMP and dCMP and use GTP as an alternate phosphate donor (PubMed:19766732, PubMed:23416111). Moreover, exhibits a diphosphate kinase activity, producing ATP, CTP, GTP, UTP, TTP, dATP, dCTP and dGTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donors (PubMed:23416111). Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK (PubMed:24767988, PubMed:26980435). Plays a protective role in the cellular response to oxidative stress (PubMed:19130895, PubMed:23474458, PubMed:26980435).
Cellular Location Mitochondrion matrix {ECO:0000255|HAMAP- Rule:MF_03170, ECO:0000269|PubMed:11485571, ECO:0000269|PubMed:19766732, ECO:0000269|PubMed:26980435}
Tissue Location Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

AK3 is a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated.

REFERENCES

Van Rompay, A.R., et al., Eur. J. Biochem. 261(2):509-517 (1999).
Yoneda, T., et al., Brain Res. Mol. Brain Res. 62(2):187-195 (1998).
Xu, G., et al., Genomics 13(3):537-542 (1992).
Robson, E.B., et al., Cytogenet. Cell Genet. 32 (1-4), 144-152 (1982).

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