GPD1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of lysate from human liver tissue lysate, using GPD1 Antibody (N-term)(Cat. #AP8507a). AP8507a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GPD1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
GPD1 Antibody (N-term) (Cat.#AP8507a) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, WB, E
Primary Accession	P21695
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	37568 Da
Antigen Region	28-57 aa

Additional Information
Gene ID	2819
Other Names	Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, GPD-C, GPDH-C, GPD1
Target/Specificity	This GPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human GPD1.
Dilution	IHC-P~~1:100~500 FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GPD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GPD1 (HGNC:4455)
Function	Has glycerol-3-phosphate dehydrogenase activity.
Cellular Location	Cytoplasm.
Tissue Location	Expressed in liver (at protein level).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.