CCHCR1 Antibody (Center) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-CCHCR1 Antibody (Center) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CCHCR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of widr cells using CCHCR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CCHCR1 Antibody (Center) (Cat. #AP8528c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green) .DAPI was used to stain the cell nuclear (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, IF, WB, E
Primary Accession	Q8TD31
Reactivity	Human
Predicted	Mouse, Rat, Bovine, Canine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	88671 Da
Antigen Region	599-627 aa

Additional Information
Gene ID	54535
Other Names	Coiled-coil alpha-helical rod protein 1, Alpha-helical coiled-coil rod protein, Putative gene 8 protein, Pg8, CCHCR1, C6orf18, HCR
Target/Specificity	This CCHCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 599-627 amino acids from the Central region of human CCHCR1.
Dilution	IHC-P~~1:100~500 FC~~1:10~50 IF~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CCHCR1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCHCR1
Synonyms	C6orf18, HCR
Function	May be a regulator of keratinocyte proliferation or differentiation.
Cellular Location	Cytoplasm. Nucleus.
Tissue Location	Found in all tissues tested, abundantly expressed in heart, liver, skeletal muscle, kidney and pancreas, and to a lesser extent in lung and placenta. Overexpressed in keratinocytes of psoriatic lesions

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.