> 首页 > 产品 > 一抗 > 癌症 > IFNB1 Antibody (N-term)

IFNB1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of IFNB1 Antibody (N-term) (Cat. #AP8539a) in HepG2 cell line lysates (35ug/lane). IFNB1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with IFNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using IFNB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	P01574
Other Accession	O77812
Reactivity	Human
Predicted	Monkey
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	22294 Da
Antigen Region	39-66 aa

Additional Information
Gene ID	3456
Other Names	Interferon beta, IFN-beta, Fibroblast interferon, IFNB1, IFB, IFNB
Target/Specificity	This IFNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of human IFNB1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	IFNB1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	IFNB1 (HGNC:5434)
Synonyms	IFB, IFNB
Function	Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli (PubMed:10049744, PubMed:10556041, PubMed:6157094, PubMed:6171735, PubMed:7665574, PubMed:8027027, PubMed:8969169). Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins (PubMed:10049744, PubMed:10556041, PubMed:7665574, PubMed:8027027, PubMed:8969169). Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways (By similarity). Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)- inducible production of tumor necrosis factor (By similarity). Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons: acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss (By similarity). IFNB1 is more potent than interferon-alpha (IFN- alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (By similarity).
Cellular Location	Secreted.