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RARS2 Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

Anti-RARS2 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of RARS2 Antibody (C-term) (Cat. #AP8590b) in HepG2 cell line lysates (35ug/lane). RARS2 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with RARS2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of NCI-H292 cells using RARS2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q5T160
Other Accession	Q0P5H7
Reactivity	Human
Predicted	Bovine, Mouse, Rat, Canine, Rabbit
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	65505 Da
Antigen Region	369-399 aa

Additional Information
Gene ID	57038
Other Names	Probable arginine--tRNA ligase, mitochondrial, Arginyl-tRNA synthetase, ArgRS, RARS2, RARSL
Target/Specificity	This RARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-399 amino acids from the C-terminal region of human RARS2.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RARS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RARS2
Synonyms	RARSL
Function	Catalyzes the attachment of arginine to tRNA(Arg) in a two- step reaction: arginine is first activated by ATP to form Arg-AMP and then transferred to the acceptor end of tRNA(Arg).
Cellular Location	Mitochondrion membrane

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

RARS2 is an arginyl-tRNA synthetase that is found in the mitochondrial matrix.

REFERENCES

Edvardson,S., et.al., Am. J. Hum. Genet. 81 (4), 857-862 (2007)
Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007)
Bonnefond,L., et.al., Biochemistry 44 (12), 4805-4816 (2005)

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¥ 1,250.00

Cat# AP8590b

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RARS2 Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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