HPD Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of HPD Antibody (C-term) (Cat. #AP8591b) in mouse liver tissue lysates (35ug/lane). HPD (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	P32754
Other Accession	P49429
Reactivity	Human
Predicted	Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	44964 Da
Antigen Region	255-284 aa

Additional Information
Gene ID	3242
Other Names	4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, HPD, PPD
Target/Specificity	This HPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from the C-terminal region of human HPD.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HPD Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	HPD
Synonyms	PPD
Function	Catalyzes the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, one of the steps in tyrosine catabolism.
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:P32755}. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P32755}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P32755}. Golgi apparatus membrane {ECO:0000250\|UniProtKB:P32755}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P32755}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.