HSPA7 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Overlay histogram showing U-2 OS cells stained with AP8748C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8748C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western blot analysis of HSPA7 Antibody (Center) (Cat. #AP8748c) in CEM cell line lysates (35ug/lane). HSPA7 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPA7 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P, E
Primary Accession	P48741
Other Accession	P17066
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	40244 Da
Antigen Region	169-196 aa

Additional Information
Other Names	Putative heat shock 70 kDa protein 7, Heat shock 70 kDa protein B, HSPA7, HSP70B
Target/Specificity	This HSPA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-196 amino acids from the Central region of human HSPA7.
Dilution	WB~~1:1000 FC~~1:25 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HSPA7 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.