IGH Antibody (C-Term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-IGH Antibody (C-Term) at 1:1000 dilution + A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of IGH Antibody (C-Term) (Cat. #AP8783b) in HL-60, A2058 cell line lysates (35ug/lane). IGH (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human skin reacted with IGH Antibody (C-Term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IGH Antibody (C-Term) (Cat. #AP8783b) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	P01876
Other Accession	P01877
Reactivity	Human
Predicted	Bovine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	290-320 aa

Additional Information
Other Names	Ig alpha-1 chain C region, IGHA1
Target/Specificity	This IGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-320 amino acids from the C-terminal region of human IGH.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	IGH Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.