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SOCS1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-SOCS1 Antibody (N-term) at 1:2000 dilution Lane 1: mouse thymus lysates Lane 2: rat spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of SOCS1 Antibody (N-term) (Cat. #AP8790a) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOCS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of SOCS1 Antibody (N-term) (Cat. #AP8790a) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
SOCS1 Antibody (N-term) (Cat. #AP8790a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

产品详情
文献引用 : 1
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, FC, E
Primary Accession	O15524
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	35-66 aa

Additional Information
Other Names	Suppressor of cytokine signaling 1, SOCS-1, JAK-binding protein, JAB, STAT-induced STAT inhibitor 1, SSI-1, Tec-interacting protein 3, TIP-3, SOCS1, SSI1, TIP3
Target/Specificity	This SOCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-66 amino acids from the N-terminal region of human SOCS1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SOCS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

SOCS1 is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling.

REFERENCES

Starr,R.,et.al., Nature 387 (6636), 917-921 (1997) Minamoto,S., et.al., Biochem. Biophys. Res. Commun. 237 (1), 79-83 (1997)

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¥ 699.00

Cat# AP8790A

Size:

25 ul

50 µl

100 µl

200 µl

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Price:

¥ 699.00

¥ 1,250.00

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SOCS1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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