- 文献引用 : 3
|Application ||IHC-P-Leica, IF, WB, FC, E|
|Other Accession||P13264, D3Z7P3|
|Reactivity||Human, Mouse, Rat|
|Other Names||Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838|
|Target/Specificity||This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GLS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.|
|Cellular Location||Isoform 1: Cytoplasm, cytosol.|
|Tissue Location||Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver Isoform 2 is expressed in cardiac and skeletal muscle|
Author : Li J1,Song P1,Jiang T2,Dai D1,Wang H1,Sun J2,Zhu L2,Xu W2,Feng L2,Shin VY3,Morrison H4,Wang X5,Jin H6.
Mol Ther. 2018 Jul 5;26(7):1828-1839. doi: 10.1016/j.ymthe.2018.04.014. Epub 2018 Apr 14.
Author : Han M1,Dai D1,Yousafzai NA1,Wang F2,Wang H1,Zhou Q1,Lu H1,Xu W2,Feng L2,Jin H2,Wang X1.
Oncotarget. 2017 Oct 6;8(61):103557-103567. doi: 10.18632/oncotarget.21581. eCollection 2017 Nov 28.
Author : Lukey MJ1, Greene KS1, Erickson JW2, Wilson KF1, Cerione RA1,2.
Nat Commun. 2016 Apr 18;7:11321. doi: 10.1038/ncomms11321.
Provided below are standard protocols that you may find useful for product applications.
Sahai (1983) demonstrated phosphate-activated glutaminase (EC 126.96.36.199) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
Swierczynski,J., et.al., Biochim. Biophys. Acta 1157 (1), 55-62 (1993)