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ALG14 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ALG14 Antibody (Center) AP8903c
    All lanes: Anti-ALG14 Antibody (Center) at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 24. 2 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ALG14 Antibody (Center) AP8903c
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALG14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - ALG14 Antibody (Center) AP8903c
    ALG14 Antibody (Center) (Cat. #AP8903c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q96F25
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 67-93 aa
Additional Information
Other Names UDP-N-acetylglucosamine transferase subunit ALG14 homolog, ALG14
Target/Specificity This ALG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the Central region of human ALG14.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALG14 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

ALG14 is involved in protein N-glycosylation. It is essential for the second step of the dolichol-linked oligosaccharide pathway. It anchors the catalytic subunit ALG13 to the ER.

REFERENCES

Gao X.-D., et.al., J. Biol. Chem. 280:36254-36262(2005).

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