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TGOLN2 Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - TGOLN2 Antibody (C-term) AP8955b
    Anti-TGOLN2 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - TGOLN2 Antibody (C-term) AP8955b
    TGOLN2 Antibody (C-term) (Cat.#AP8955b) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession O43493
Other Accession P19814, Q62314, Q62313
Reactivity Human
Predicted Mouse, Rat, Bovine, Canine, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 45880 Da
Antigen Region 399-427 aa
Additional Information
Gene ID 10618
Other Names Trans-Golgi network integral membrane protein 2, TGN38 homolog, TGN46, TGN48, Trans-Golgi network protein TGN51, TGOLN2, TGN46, TGN51
Target/Specificity This TGOLN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 amino acids from the C-terminal region of human TGOLN2.
Dilution FC~~1:10~50
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTGOLN2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name TGOLN2 (HGNC:15450)
Synonyms TGN46, TGN51
Function May be involved in regulating membrane traffic to and from trans-Golgi network.
Cellular Location Cell membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Note=Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes
Tissue Location Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells
Research Areas

BACKGROUND

TGOLN2 may be involved in regulating membrane traffic to and from trans-Golgi network.

REFERENCES

Wu,C., et.al., Proteomics 7 (11), 1775-1785 (2007)
Honing,S., et.al., Mol. Cell 18 (5), 519-531 (2005)

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