beta Tubulin Antibody

Rabbit mAb

Western blot analysis of beta Tubulin expression in (1) MCF-7 cell lysate; (2) COS-1 cell lysate; (3) Jurkat cell lysate; (4) HeLa cell lysate.
Immunohistochemical analysis of paraffin-embedded (1) Human glioma; (2) Rat brain; (3) Mouse kidney; (4) Human uterus cancer, using beta Tubulin Antibody.
Immunofluorescent analysis of NIH/3T3 cells, using beta Tubulin Antibody .
Remifentanil upregulates hepatic IL-18 binding protein (IL-18BP) expression through transcriptional control. -Laboratory Investigation
Polysaccharides from Nostoc commune Vaucher activate macrophages via NF-κB and AKT/JNK1/2 pathways to suppress colorectal cancer growth in vivo. -Food & Function

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC, ICC, IHF
Primary Accession	P07437
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	Beta 4 tubulin; Beta 5 tubulin; Beta1 tubulin; Tubulin beta chain; Tubulin beta class I;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	49671 Da

Additional Information
Dilution	WB 1:3000~1:20000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:100
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human beta Tubulin
Description	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TUBB
Synonyms	TUBB5
Function	Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location	Cytoplasm, cytoskeleton
Tissue Location	Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.