ER alpha Antibody

Rabbit mAb

Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate.
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody.
Immunofluorescent analysis of MCF7 cells, using ER alpha Antibody .

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC, ICC, IP, IHF
Primary Accession	P03372
Reactivity	Rat, Human, Mouse, Dog
Clonality	Monoclonal
Other Names	ER; ESR; ESR1; Era; ESRA; NR3A1;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	60 KDa

Additional Information
Dilution	WB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 ChIP 5 ?g/30 ?g FC 1:30
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human ER alpha
Description	ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.