HMGB1 Antibody

Rabbit mAb

Western blot analysis of HMGB1 expression in (1)HeLa cell lysate; (2)Rat brain lysate.
Immunohistochemical analysis of paraffin-embedded human kidney, using HMGB1 Antibody.
Immunofluorescent analysis of SW480 cells, using HMGB1 Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC, ICC, IHF
Primary Accession	P09429
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	HMGB1; HMG3; HMG1; SBP 1; Amphoterin;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	25 KDa

Additional Information
Dilution	WB 1:1000~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 FC 1:30
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human HMGB1
Description	High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFκB family, ATF-2 and c-Jun to activate transcription.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.