Vimentin Antibody

Rabbit mAb

Western blot analysis of Vimentin expression in HEK293 cell lysate.
Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody.
Immunofluorescent analysis of Hela cells, using Vimentin Antibody.
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry
Overexpression of the 14-3-3γ protein in uterine leiomyoma cells results in growth retardation and increased apoptosis. -Cellular Signalling

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC, ICC, IHF
Primary Accession	P08670
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	FLJ36605; VIM; VIME; Vimentin
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	53652 Da

Additional Information
Dilution	WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:30
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human Vimentin
Description	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	VIM (HGNC:12692)
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).
Cellular Location	Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250\|UniProtKB:P31000}. Cell membrane {ECO:0000250\|UniProtKB:P20152}
Tissue Location	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Research Areas