Nanog Antibody

Rabbit mAb

Western blot analysis of Nanog expression in NCCIT cell lysate.
Immunohistochemical analysis of paraffin-embedded rat testis, using Nanog Antibody.
Immunofluorescent analysis of NCCIT cells, using Nanog Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC, ICC, IHF
Primary Accession	Q9H9S0
Reactivity	Human
Clonality	Monoclonal
Other Names	Homeobox transcription factor Nanog
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	37 KDa

Additional Information
Dilution	WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human Nanog
Description	Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Acts as a transcriptional activator or repressor By similarity.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.