Cytochrome C Antibody

Rabbit mAb

Western blot analysis of Cytochrome C expression in Human kidney lysate.
Immunohistochemical analysis of paraffin-embedded human liver, using Cytochrome C Antibody.
Immunofluorescent analysis of Hela cells, using Cytochrome C Antibody.
Taurine protects INS-1 cells from apoptosis induced by Di(2-ethylhexyl) phthalate via reducing oxidative stress and autophagy. -Toxicology Mechanisms and Methods
Pyrroloquinoline quinine ameliorates doxorubicin-induced autophagy-dependent apoptosis via lysosomal-mitochondrial axis in vascular endothelial cells. -Toxicology
Mechanistic insights into geniposide regulation of bile salt export pump (BSEP) expression. -RSC Advances

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP, IHF
Primary Accession	P99999
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	CYC; CYCS; Cytochrome c; HCS; THC4;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	11749 Da

Additional Information
Dilution	WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human Cytochrome C
Description	Cytochrome C is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome C into the cytosol.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	CYCS
Synonyms	CYC
Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Cellular Location	Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane

Research Areas