NOX2/CYBB/gp91phox Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P04839
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	CGD; CGD91-phox; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	65336 Da

Additional Information
Dilution	WB 1:1000~1:2000
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human NOX2/CYBB/gp91phox
Description	The superoxide-generating NADPH oxidase complex expresses in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	CYBB (HGNC:2578)
Synonyms	NOX2
Function	Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:15338276, PubMed:36241643, PubMed:36413210, PubMed:38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (Probable) (PubMed:38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed:19028840, PubMed:38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (By similarity). NADPH oxidase complex assembly is impaired through interaction with NRROS (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein. Note=As unassembled monomer may localize to the endoplasmic reticulum
Tissue Location	Detected in neutrophils (at protein level).