CDC34 Antibody

Rabbit mAb

Western blot analysis of CDC34 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.
Immunofluorescent analysis of Hela cells, using CDC34 Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, FC, ICC, IP
Primary Accession	P49427
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	CDC34; Cell division cycle 34; UBC3; UBCH3; Ubiquitin-protein ligase R1; UBE2R1; E2-CDC34; Ubiquitin carrier protein;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	35 KDa

Additional Information
Dilution	WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human CDC34
Description	Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.