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|Application ||IF, WB, FC, E|
|Other Accession||Q8K585, P17095, Q9QXP3|
|Calculated MW||11676 Da|
|Other Names||High mobility group protein HMG-I/HMG-Y, HMG-I(Y), High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein R, HMGA1, HMGIY|
|Target/Specificity||This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HMGA1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.|
|Cellular Location||Nucleus. Chromosome.|
Provided below are standard protocols that you may find useful for product applications.
HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.
Mu,G., et.al., Hum. Pathol. 41 (4), 493-502 (2010)
Kim,J.J., et.al., J. Hum. Genet. 55 (1), 27-31 (2010)