CD59 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, FC, ICC, IP
Primary Accession	P13987
Reactivity	Human
Clonality	Monoclonal
Other Names	CD59 glycoprotein; HRF20; MAC-IP; MEM43 antigen; MACIF; MIRL; Protectin; MIC11; MIN1; p18 20;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	14177 Da

Additional Information
Dilution	WB 1:1000~1:5000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human CD59
Description	CD59 is a GPI-anchored glycoprotein that is expressed on leukocytes, vascular endothelial cells, various epithelial cells and placenta. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	CD59 {ECO:0000303\|PubMed:2475570, ECO:0000312\|HGNC:HGNC:1689}
Function	Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed:11882685, PubMed:1698710, PubMed:2475111, PubMed:2475570, PubMed:2606909, PubMed:9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed:11882685, PubMed:1698710, PubMed:36797260).
Cellular Location	Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Localizes to the cell surface (PubMed:36797260). Soluble form found in a number of tissues (PubMed:8670172).