TLR5 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	O60602
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	SLEB1; TIL3; Tlr5; Toll like receptor 5 precursor;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	97800 Da

Additional Information
Dilution	WB 1:500~1:2000
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human TLR5
Description	Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities and designated toll-like receptors (TLR). Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	TLR5
Synonyms	TIL3
Function	Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response (PubMed:11323673, PubMed:18490781). Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota (PubMed:29934223). Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF- kappa-B activation, cytokine secretion and induction of the inflammatory response (PubMed:11489966, PubMed:20855887). Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Highly expressed on the basolateral surface of intestinal epithelia (PubMed:11489966). Expressed also in other cells such as lung epithelial cells (PubMed:11489966, PubMed:18490781)