CD40L Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P29965
Reactivity	Human, Mouse
Clonality	Monoclonal
Other Names	CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40LG; CD40L; TNFSF5; TRAP;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	29274 Da

Additional Information
Dilution	WB 1:500~1:2000
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human CD40L
Description	Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	CD40LG
Synonyms	CD40L, TNFSF5, TRAP
Function	Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).
Cellular Location	Cell membrane; Single-pass type II membrane protein. Cell surface
Tissue Location	Specifically expressed on activated CD4+ T- lymphocytes