CHD4 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, FC, ICC
Primary Accession	Q14839
Reactivity	Human
Clonality	Monoclonal
Other Names	ATP dependent helicase CHD4; CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	218005 Da

Additional Information
Dilution	WB 1:1000~1:5000 ICC/IF 1:50~1:200 FC 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human CHD4
Description	Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	CHD4
Function	ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA (PubMed:28977666, PubMed:32543371). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:17626165, PubMed:28977666, PubMed:9804427). Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination (PubMed:25593309). Involved in neurogenesis (By similarity).
Cellular Location	Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrosomes in interphase (By similarity). Localizes to sites of DNA damage in a manner dependent on ZMYND8 and ZNF687 (PubMed:27732854, PubMed:28977666). {ECO:0000250\|UniProtKB:Q6PDQ2, ECO:0000269\|PubMed:27732854, ECO:0000269\|PubMed:28977666}
Tissue Location	Widely expressed..