Cyclophilin F Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP
Primary Accession	P30405
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	Cyclophilin 3; cyclophilin D; Cyclophilin F; Cyp D; CyP M; CYP3; CypD; hCyP3; Mitochondrial cyclophilin; PPIase; PPIase F; Ppif; Rotamase;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	22040 Da

Additional Information
Dilution	WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human Cyclophilin F
Description	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	PPIF
Synonyms	CYP3
Function	PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Involved in regulation of the mitochondrial permeability transition pore (mPTP) (PubMed:26387735). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated (PubMed:26387735). In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis (PubMed:22726440). Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels (By similarity). Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis (PubMed:19228691).
Cellular Location	Mitochondrion matrix