CD5 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP, IHF
Primary Accession	P06127
Reactivity	Human
Clonality	Monoclonal
Other Names	CD5; CD5 antigen (p56 62); CD5 molecule; LEU1; LyA; Lymphocyte antigen T1/Leu-1; T-cell surface glycoprotein CD5; T1;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	54578 Da

Additional Information
Dilution	WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human CD5
Description	May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	CD5
Synonyms	LEU1
Function	Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed:1384049, PubMed:1385158). Mechanistically, co- engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed:23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed:27499044).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P13379}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P13379}