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KIR3DL3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - KIR3DL3 Antibody (Center) AP9170c
    Western blot analysis of KIR3DL3 Antibody (Center) (Cat. #AP9170c) in K562 cell line lysates (35ug/lane). KIR3DL3 (arrow) was detected using the purified Pab.
  • 14 - KIR3DL3 Antibody (Center) AP9170c
    Formalin-fixed and paraffin-embedded human tonsil reacted with KIR3DL3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - KIR3DL3 Antibody (Center) AP9170c
    KIR3DL3 Antibody (Center) (Cat. #AP9170c) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q8N743
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44928 Da
Antigen Region 132-158 aa
Additional Information
Other Names Killer cell immunoglobulin-like receptor 3DL3, CD158 antigen-like family member Z, Killer cell inhibitory receptor 1, CD158z, KIR3DL3, CD158Z, KIR3DL7, KIRC1
Target/Specificity This KIR3DL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-158 amino acids from the Central region of human KIR3DL3.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKIR3DL3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KIR3DL3
Synonyms CD158Z, KIR3DL7, KIRC1
Function Receptor on natural killer cells. May inhibit the activity of NK cells thus preventing cell lysis.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KIR3DL3 is transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. This protein are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC).

REFERENCES

Trompeter,H.I., et.al., J. Immunol. 174 (7), 4135-4143 (2005)
Arnheim,L., et.al., Tissue Antigens 65 (3), 252-259 (2005)

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