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>   首页   >   产品   >   重组兔单克隆抗体   >   重组兔单克隆抗体   >   eIF3e Antibody   

eIF3e Antibody

Rabbit mAb

     
  • 1 - eIF3e Antibody AP92519
    Western blot analysis of eIF3e expression in (1) 293T cell lysate; (2) Jurkat cell lysate.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, ICC
Primary Accession P60228
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Other Names eIF3e; EIF3S6; eIFe;
Isotype Rabbit IgG
Host Rabbit
Calculated MW 52221 Da
Additional Information
Dilution WB 1:500~1:2000 ICC/IF 1:50~1:200
Purification Affinity-chromatography
Immunogen A synthesized peptide derived from human eIF3e
Description Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
Storage Condition and Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Protein Information
Name EIF3E {ECO:0000255|HAMAP-Rule:MF_03004}
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl- tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed:17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed:17310990, PubMed:17324924).
Cellular Location Cytoplasm. Nucleus, PML body.
Tissue Location Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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