BTLA (6U2) Mouse Monoclonal Antibody

25 ng of recombinant human BTLA was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. AP93636 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. BTLA band was visualized using ECL Substrate. Result: AP93636 can detect human BTLA by Western blotting.
IHC-P analysis of human tonsil tissue by anti-human BTLA antibody (AP93636). IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human BTLA primary antibody (AP93636) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Non-germinal center cells and a few of germinal center cells are positively stained at the cell membrane.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q7Z6A9-2
Reactivity	Human
Clonality	Monoclonal