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HPV16 E7 Rabbit pAb

HPV16 E7 Rabbit pAb

     
  • 0 - HPV16 E7 Rabbit pAb AP94081
    Blank control（black line）:Hela.
    Primary Antibody (green line): Rabbit Anti-HPV16 E7 antibody (AP94081)
    Dilution:1ug/Test;
    Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
    Dilution: 0.5ug/Test.
    Isotype control（orange line）: Normal Rabbit IgG
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
  • 1 - HPV16 E7 Rabbit pAb AP94081
    Sample:
    Lane 1: HPV16 E7 fusion protein (AP94081) 0.1ug Lysates
    Lane 2: HPV16 E7 fusion protein (AP94081) 0.05ug Lysates


    Primary: Anti-HPV16 E7 (AP94081) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 11kDa
    Observed band size: 50kDa
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Reactivity HPV16
Host Rabbit
Clonality Polyclonal
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from HPV16 E7 protein
Epitope Specificity 11-70/98
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Additional Information
Dilution WB=1:500-2000,Flow-Cyt=1ug/Test
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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¥ 1,250.00
Cat# AP94081
Size:
50 µl
100 µl
Price:
¥ 1,250.00
¥ 2,050.00
Quantity:
reduce add
reduce add
Availability: 3-5 days
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