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Strep-Tag II Mouse mAb

Strep-Tag II Mouse mAb

     
  • 1 - Strep-Tag II Mouse mAb AP94084
    Sample:
    Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysates (AP94084)
    Lane 2: Negative control
    Primary: Anti-Strep-Tag II/HRP (AP94084-HRP) at 1/10000 dilution
    Predicted band size: kDa
    Observed band size: 51 kDa
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF
Host Mouse
Clonality Monoclonal
Physical State Liquid
Immunogen KLH conjugated synthetic peptide Strep-Tag
Isotype IgG
Purity affinity purified by Protein G
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
Additional Information
Dilution WB=1:1000-5000,ELISA=1:1000-5000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,ICC/IF=1:100-500,Flow-Cyt=1ug/Test
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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